SubtiBank SubtiBank
Version comparison:

2017-05-24 16:11:312025-05-12 05:47:12

locus

BSU09990

BSU_09990

geneLength

609

612

product

transcriptional repressor (MarR family)

transcriptional repressor ([SW|MarR family])

ec

outlinks

bsu

BSU09990

BSU_09990

The protein

Effectors of protein activity

activity is inhibited in an unknown way by [[protein|PrkA]] (possibly phosphorylation) [Pubmed|25983726]

activity is inhibited in an unknown way by [[protein|PrkA]] (possibly phosphorylation) [Pubmed|25983726]

DNA binding is affected by [[protein|DnmA]]-mediated DNA methylation [pubmed|32324221]

Biological materials

Mutant

1A918 ( ''scoC''::''erm trpC2 leuC7''), [Pubmed|15126467], available at the [http://bgsc.org/getdetail.php?bgscid=1A918 Bacillus Genetic Stock Center]

BKE09990 ( ''scoC''::''erm trpC2'') is available at the [http://bgsc.org/ Bacillus Genetic Stock Center]

1A918 ( ''scoC''::''erm trpC2 leuC7''), [Pubmed|15126467], available at the [http://bgsc.org/getdetail.php?bgscid=1A918 Bacillus Genetic Stock Center]

BKE09990 (Δ[[gene|scoC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA

BKK09990 (Δ[[gene|scoC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA

Biological materials

Expression vector

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

References

3131303, 16923912, 1906467, 10383984, 16321961, 14629015, 1907636, 15126467, 2504584, 15104138, 19118355, 19801406, 19898538, 20382764, 22517742, 23660663, 11717292, 25966844, 19251843, 26473603, 25983726, 26728191

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|HTH marR-type domain] (aa 13-157) (according to UniProt)

The protein

additional information

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000050 PRODORIC2 database]

Biological materials

Expression vectors

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab

References

Reviews

28886686

References

Original publications

3131303, 16923912, 1906467, 10383984, 16321961, 14629015, 1907636, 15126467, 2504584, 15104138, 19118355, 19801406, 19898538, 20382764, 22517742, 23660663, 11717292, 25966844, 19251843, 26473603, 25983726, 26728191, 31039790, 32324221