2017-05-24 16:11:312025-05-12 05:47:12
locus
BSU09990
BSU_09990
geneLength
609
612
product
transcriptional repressor (MarR family)
transcriptional repressor ([SW|MarR family])
ec
outlinks
bsu
BSU09990
BSU_09990
The protein
Effectors of protein activity
activity is inhibited in an unknown way by [[protein|PrkA]] (possibly phosphorylation) [Pubmed|25983726]
activity is inhibited in an unknown way by [[protein|PrkA]] (possibly phosphorylation) [Pubmed|25983726]
DNA binding is affected by [[protein|DnmA]]-mediated DNA methylation [pubmed|32324221]
Biological materials
Mutant
1A918 ( ''scoC''::''erm trpC2 leuC7''), [Pubmed|15126467], available at the [http://bgsc.org/getdetail.php?bgscid=1A918 Bacillus Genetic Stock Center]
BKE09990 ( ''scoC''::''erm trpC2'') is available at the [http://bgsc.org/ Bacillus Genetic Stock Center]
1A918 ( ''scoC''::''erm trpC2 leuC7''), [Pubmed|15126467], available at the [http://bgsc.org/getdetail.php?bgscid=1A918 Bacillus Genetic Stock Center]
BKE09990 (Δ[[gene|scoC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA
BKK09990 (Δ[[gene|scoC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA
Biological materials
Expression vector
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
References
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|HTH marR-type domain] (aa 13-157) (according to UniProt)
The protein
additional information
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000050 PRODORIC2 database]
Biological materials
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
References
Reviews
References
Original publications